›› 2011, Vol. 23 ›› Issue (6): 446-448.doi: 10.3969/j.issn.1004-616x.2011.06.010

• 核辐射安全专家论坛 • Previous Articles     Next Articles

Feasibility of rapidly estimating biological dose under shortened incubation of premature chromosome condensation rings

WANG Chuan-gao1，2，WANG Zhi-dong，CHEN Mao-sheng，DU Jie，ZHANG Xue-qing，WANG Zhong-wen，CHEN Ying   

  1. 1. Department of Radiation Toxicology and Oncology, Institute of Radiation Medicine, Beijing 100850; 2. China Institute of Atomic Energy, Beijing 102413, China
  • Received:2011-09-19 Revised:2011-09-22 Online:2011-11-30 Published:2011-11-30
  • Contact: WANG Zhi- dong

PDF (PC)

993

Abstract: OBJECTIVE: To explore the feasibility of rapidly estimating biological dose under shortened incubation time and establish dose-effect curve of premature chromosome condensation rings (PCC-R) in human lymphocytes induced by γ-rays. METHODS： Human peripheral blood was irradiated in vitro by 60Co γ- rays at 0，1，3，6，10，15 and 20 Gy. PCC-R was counted after 44 h and 48 h incubation with Okadaic acid added 1 h before harvest. Both 44 h and 48 h dose-effect curves were established and compared. RESULTS：Cells obtained from 44 h incubation was slightly decreased，and PCC rings rate was slightly lowered compared with 48 h incubation ( P>0.05). PCC rings rate increased with dose and demonstrated a perfect linear relationship (0-15 Gy) ，with a linear equation Y= - 0.015 2+ 0.062 5D (R2=0.983 9). CONCLUSION：After shortening the incubation time to 44 h，PCC rings could still be used in dose estimation of high dose radiation incidents within 15 Gy.

Key words: high dose irradiation, premature chromosome condensation rings, shortened incubation time, dose-effect curves